Nucleic Acid Amplification Life Science Dashboard™ Series 3

Published by: Percepta Associates, Inc.

Published: Sep. 1, 2011 - 204 Pages

Table of Contents

Figures and Tables

Executive Summary

Key Findings and Implications

Nucleic Acid Amplification Dashboard

Nucleic Acid Amplification Market Opportunity Matrix

Survey Methodology

Survey Invitation Text

Respondent Demographics

Frequency of Performance of Life Science Techniques

Frequency of Performance of Nucleic Acid Amplification

Reaction Throughput and Market Growth Rates

Respondents’ Stated Price Per Reaction

Total Market Size, Market Segment Sizes and Total Market Growth Rate

Market Shares by Segment (Share of Mentions)

Customer Satisfaction And Interest In Switching Suppliers

Product Features That Influence Purchasing Decisions

Primary and Secondary Downstream Applications

Desired Changes to Nucleic Acid Amplification Products

Survey Questionnaire

Appendix

Figures and Tables

List of Tables

Figure 1: Respondents’ Place of Employment

Figure 2: Respondent’s Location

Figure 3: Respondents’ Job Title

Figure 4: Respondents’ Areas of Expertise/Specialization

Figure 5: Number of Employees in Respondents’ Laboratories

Figure 6: Percentage of Respondents Performing Various Techniques at Least a Few Times per Year

Figure 7: Percentage of Respondents Performing Nucleic Acid Amplification

Figure 7A: Change in Percentage of Respondents Performing Nucleic Acid Amplification

Figure 8: Percentage of Respondents Performing Various Nucleic Acid Amplification Techniques at Least a Few Times per Year

Figure 9: Percentage of Respondents Using Various Thermostable Polymerases for Nucleic Acid Amplification Techniques

Figure 10: Percentage of Respondents Performing Difficult PCR

Figure 11: Percentage of Respondents Performing Digital PCR

Figure 12: Percentage of Respondents Performing Emulsion PCR

Figure 13: Percentage of Respondents Performing Fast PCR

Figure 14: Percentage of Respondents Performing High Fidelity PCR

Figure 14A: Change in Percentage of Respondents Performing High Fidelity PCR

Figure 15: Percentage of Respondents Performing High Resolution Melt

Figure 16: Percentage of Respondents Performing Standard Endpoint PCR

Figure 17: Percentage of Respondents Performing Standard Endpoint RT-PCR

Figure 18: Percentage of Respondents Performing qPCR

Figure 19: Percentage of Respondents Performing qRT-PCR

Figure 20: Respondents’ Primary Supplier for Difficult PCR

Figure 21: Respondents’ Primary Supplier for Digital PCR

Figure 22: Respondents’ Primary Supplier for Emulsion PCR

Figure 23: Respondents’ Primary Supplier for Fast PCR

Figure 24: Respondents’ Primary Supplier for High Fidelity PCR

Figure 24A: Change in Mention for Respondents’ Primary Supplier for High Fidelity PCR

Figure 25: Respondents’ Primary Supplier for High Resolution Melt

Figure 26: Respondents’ Primary Supplier for Standard Endpoint PCR

Figure 27: Respondents’ Primary Supplier for Standard Endpoint RT-PCR

Figure 28: Respondents’ Primary Supplier for qPCR

Figure 29: Respondents’ Primary Supplier for qRT-PCR

Figure 30: Respondents’ Primary Supplier of Instruments for Standard PCR

Figure 30A: Change in Respondents’ Primary Supplier of Instruments for Standard PCR

Figure 31: Respondents’ Primary Supplier of Instruments for Real Time PCR

Figure 31A: Change in Respondents’ Primary Supplier of Instruments for Real Time PCR

Figure 32: Respondents’ Primary and Secondary Applications for End Products from Difficult PCR

Figure 33: Respondents’ Primary and Secondary Applications for End Products from Digital PCR

Figure 34: Respondents’ Primary and Secondary Applications for End Products from Emulsion PCR

Figure 35: Respondents’ Primary and Secondary Applications for End Products from Fast PCR

Figure 36: Respondents’ Primary and Secondary Applications for End Products from High Fidelity PCR

Figure 37: Respondents’ Primary and Secondary Applications for End Products from High Resolution Melt

Figure 38: Respondents’ Primary and Secondary Applications for End Products from Standard Endpoint PCR

Figure 39: Respondents’ Primary and Secondary Applications for End Products from Standard Endpoint RT-PCR

Figure 40: Respondents’ Primary and Secondary Applications for End Products from qPCR

Figure 41: Respondents’ Primary and Secondary Applications for End Products from qRT-PCR

Figure 42: Non current users of Nucleic Acid Amplification Products - Future Plans

List of tables

Table 1: Respondents’ Area of Expertise (Values for Figure 4)

Table 2: Frequency of Performance of Various Life Science Techniques

Table 3: Frequency of Co-Performance of Various Life Science Techniques

Table 4: Percentage of Respondents Performing Nucleic Acid Amplification by Place of Employment and Location

Table 5: Frequency of Performance of Various Nucleic Acid Amplification Techniques

Table 6: Frequency of Co-Performance of Life Science Techniques with Nucleic Acid Amplification Techniques

Table 7: Frequency of Co-Performance of Nucleic Acid Amplification Techniques with Life Science Techniques

Table 8: Mean, Median and Trim Mean Monthly Throughput for Nucleic Acid Amplification Techniques

Table 8A: Change in Median Monthly Throughput for Nucleic Acid Amplification Methods - Comparison to the 2009 Dashboard

Table 8B: Mean, Median and Trim Mean Monthly Throughput for Nucleic Acid Amplification Methods by Organization Type

Table 9: Percentage of Respondents Performing Probe-based vs. DNA Binding-dye based Reactions for real time PCR

Table 10: Percentage of Respondents Performing Various Numbers of Amplification Reactions Per Month by Product Category

Table 11: Projected Growth in the Performance of Various Nucleic Acid Amplification Techniques

Table 12: Mean, Median and Trim Mean Price Per Reaction for Nucleic Acid Amplification Techniques

Table 13: Change in Mean, Median and Trim Mean Price Per Reaction for Nucleic Acid Amplification Techniques

Table 14: Estimated 2012 Global Market Size for Nucleic Acid Amplification Techniques

Table 15: Respondents’ Primary Supplier for Difficult PCR by Market Segment

Table 16: Respondents’ Primary Supplier for Digital PCR by Market Segment

Table 17: Respondents’ Primary Supplier for Emulsion PCR by Market Segment

Table 18: Respondents’ Primary Supplier for Fast PCR by Market Segment

Table 19: Respondents’ Primary Supplier for High Fidelity PCR by Market Segment

Table 20: Respondents’ Primary Supplier for High Resolution Melt by Market Segment

Table 21: Respondents’ Primary Supplier for Standard Endpoint PCR by Market Segment

Table 22: Respondents’ Primary Supplier for Standard Endpoint RT-PCR by Market Segment

Table 23: Respondents’ Primary Supplier for qPCR by Market Segment

119 Table 24: Respondents’ Primary Supplier for qRT-PCR by Market Segment

120 Table 25: Market Share Leaders for Nucleic Acid Amplification Products

125 Table 26: Respondents’ Primary Supplier of Standard PCR Thermocyclers by Market Segment

131 Table 27: Respondents’ Primary Supplier for Instruments for Real Time PCR by Market Segment

132 Table 28: Market Share Leaders for Standard and Real Time PCR Instruments

135 Table 29: Percentage of Respondents Satisfied with Various Nucleic Acid Amplification Techniques and Reasons for Dissatisfaction

137 Table 30: Percentage of Respondents That Have Switched Suppliers in the Last Six Months

140 Table 31: Most Important Features of Nucleic Acid Amplification Products

141 Table 31A: Most Important Features of Nucleic Acid Amplification Products - Comparison to 2009 Nucleic Acid Amplification Dashboard

145 Table 32: Respondents’ Primary and Secondary Applications for End Products from Difficult PCR (Values for Figure 32)

149 Table 33: Respondents’ Primary and Secondary Applications for End Products from Digital PCR (Values for Figure 33)

152 Table 34: Respondents’ Primary and Secondary Applications for End Products from Emulsion PCR (Values for Figure 34)

155 Table 35: Respondents’ Primary and Secondary Applications for End Products from Fast PCR (Values for Figure 35)

160 Table 36: Respondents’ Primary and Secondary Applications for End Products from High Fidelity PCR (Values for Figure 36)

163 Table 37: Respondents’ Primary and Secondary Applications for End Products from High Resolution Melt (Values for Figure 37)

167 Table 38: Respondents’ Primary and Secondary Applications for End Products from Standard Endpoint PCR (Values for Figure 38)

171 Table 39: Respondents’ Primary and Secondary Applications for End Products from Standard Endpoint RT-PCR (Values for Figure 39)

175 Table 40: Respondents’ Primary and Secondary Applications for End Products from qPCR (Values for Figure 40)

179 Table 41: Respondents’ Primary and Secondary Applications for End Products from qRT-PCR (Values for Figure 41)

181 Table 42: Respondents’ Primary Applications for End Products from Nucleic Acid Amplification

Abstract

• Difficult PCR (e.g. GC rich, complex, long targets)
• Digital PCR
• Emulsion PCR
• Fast PCR
• High Fidelity PCR
• High Resolution Melt (using real time PCR instrument)
• Standard endpoint PCR
• Standard endpoint RT-PCR
• qPCR (real time PCR with genomic DNA template)
• qRT-PCR (real time PCR with RNA or cDNA template)

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