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Nucleic Acid Amplification Life Science Dashboard™ Series 2

Published by: Percepta Associates, Inc.

Published: Jul. 1, 2009 - 169 Pages


Table of Contents



Figures and Tables

Executive Summary

Key Findings and Implications

Nucleic Acid Amplification Dashboard

Nucleic Acid Amplification Market Opportunity Matrix

Survey Methodology

Survey Invitation Text

Respondent Demographics

Frequency of Performance of Molecular Biology Techniques

Frequency of Performance of Nucleic Acid Amplification

Reaction Throughput and Market Growth Rates

Respondent’s Stated Price Per Reaction

Total Market Size, Market Segment Sizes and Total Market Growth Rate

Market Shares by Segment (Share of Mention)

Customer Satisfaction and Interest In Switching Suppliers

Product Features That Influence Purchasing Decisions

Primary and Secondary Downstream Applications

Desired Changes to Nucleic Acid Amplification Products

Survey Questionnaire


Figures and Tables

Figure 1: Respondent’s Place of Employment

Figure 2: Respondent’s Country/Region

Figure 3: Respondent’s Job Title

Figure 4: Respondent’s Areas of Expertise/Specialization

Figure 5: Number of Employees in Respondent’s Laboratories

Figure 6: Percentage of Respondents Performing Various Techniques at Least a Few Times per Year

Figure 7: Percentage of Respondents Performing Nucleic Acid Amplification

Figure 8: Percentage of Respondents using Various Nucleic Acid Amplification Techniques in their Laboratory

Figure 9: Percentage of Respondents Performing Standard PCR with Taq Polymerase

Figure 10: Percentage of Respondents Performing Standard PCR with a Hot Start Taq Polymerase

Figure 11: Percentage of Respondents Performing Standard PCR with a Proofreading (High Fidelity) Polymerase

Figure 12: Percentage of Respondents Performing qPCR (Genomic DNA Template) Using Taq Polymerase

Figure 13: Percentage of Respondents Performing qPCR (Genomic DNA Template) Using Hot Start Taq Polymerase

Figure 14: Percentage of Respondents Performing qRT-PCR (cDNA Template) Using Taq Polymerase

Figure 15: Percentage of Respondents Performing qRT-PCR (cDNA Template) Using Hot Start Taq Polymerase

Figure 16: Respondent’s Primary Supplier for Standard PCR with Taq Polymerase

Figure 17: Respondent’s Primary Supplier for Standard PCR with a Hot Start Taq Polymerase

Figure 18: Respondent’s Primary Supplier for Standard PCR with a Proofreading (High Fidelity) Polymerase

Figure 19: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Taq Polymerase

Figure 20: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Hot Start Taq Polymerase

Figure 21: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Taq Polymerase

Figure 22: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Hot Start Taq Polymerase

Figure 23: Respondent’s Primary Supplier of Standard PCR Thermocyclers

Figure 24: Respondent’s Primary Supplier of Instruments for Real-Time PCR

Figure 25: Percentage of Respondents That Have Switched Suppliers in the Last Six Months

Figure 26: Most Important Features of Nucleic Acid Amplification Products

Figure 27: Respondent’s Primary Application for End Products from Standard PCR with Taq Polymerase

Figure 28: Respondent’s Primary & Secondary Application for End Products from Standard PCR with Taq Polymerase

Figure 29: Respondent’s Primary Application for End Products from Standard PCR with a Hot Start Taq Polymerase

Figure 30: Respondent’s Primary & Secondary Application for End Products from Standard PCR with a Hot Start Taq Polymerase

Figure 31: Respondent’s Primary Application for End Products from Standard PCR with a Proofreading (High Fidelity) Polymerase

Figure 32: Respondent’s Primary & Secondary Application for End Products from Standard PCR with a Proofreading (High Fidelity) Polymerase

Figure 33: Respondent’s Primary Application for End Products from qPCR (genomic DNA Template) using Taq Polymerase

Figure 34: Respondent’s Primary & Secondary Application for End Products from qPCR (genomic DNA Template) using Taq Polymerase

Figure 35: Respondent’s Primary Application for End Products from qPCR (genomic DNA Template) using a Hot Start Taq Polymerase

Figure 36: Respondent’s Primary & Secondary Application for End Products from qPCR (genomic DNA Template) using a Hot Start Taq Polymerase

Figure 37: Respondent’s Primary Application for End Products from qRT-PCR (cDNA Template) using Taq Polymerase

Figure 38: Respondent’s Primary & Secondary Application for End Products from qRT-PCR (cDNA Template) using Taq Polymerase

Figure 39: Respondent’s Primary Application for End Products from qRT-PCR (cDNA Template) using a Hot Start Taq Polymerase

Figure 40: Respondent’s Primary & Secondary Application for End Products from qRT-PCR (cDNA Template) using a Hot Start Taq Polymerase




Table 1: Respondent’s Areas of Expertise/Specialization Values for Figure 4

Table 2: Frequency of Performance of Various Life Science Techniques

Table 3: Frequency of Co-Performance of Various Life Science Techniques

Table 4: Frequency of use for Various Nucleic Acid Amplification Techniques

Table 5: Frequency of Co-Performance of Life Science Techniques with the Use of Various Nucleic Acid Amplification Products

Table 6: Frequency of Use of Nucleic Acid Amplification Products with the Performance of Life Science Techniques

Table 7: Median and Average Monthly Throughput for Nucleic Acid Amplification Products

Table 8: Percentage of Respondents Performing Various Numbers of Amplification Reactions Per Month by Product Category

Table 9: Projected Growth in the Use of Various Nucleic Acid Amplification Product Categories

Table 10: Median and Average Price Per Amplification for Nucleic Acid Amplification Products

Table 11: Estimated 2009 Global Market Size for Nucleic Acid Amplification Products by Category

Table 12: Respondent’s Primary Supplier for Standard PCR with Taq Polymerase by Market Segment

Table 13: Respondent’s Primary Supplier for Standard PCR with a Hot Start Taq Polymerase by Market Segment

Table 14: Respondent’s Primary Supplier for Standard PCR with a Proofreading (High Fidelity) Polymerase by Market Segment

Table 15: Market Share Leaders for Standard PCR Amplification Products

Table 16: Respondent’s Primary Supplier for qPCR (Genomic DNA Template) using Taq Polymerase by Market Segment

Table 17: Respondent’s Primary Supplier for qPCR (Genomic DNA Template) using Hot Start Taq Polymerase by Market Segment

Table 18: Market Share Leaders for qPCR (Genomic DNA) Amplification Products

Table 19: Respondent’s Primary Supplier for qRT-PCR (cDNA template) using Taq Polymerase by Market Segment

Table 20: Respondent’s Primary Supplier for qRT-PCR (cDNA template) using a Hot Start Taq Polymerase by Market Segment

Table 21: Market Share Leaders for RT-PCR (cDNA) Amplification Products

Table 22: Respondent’s Primary Supplier for Standard PCR Thermocyclers by Market Segment

Table 23: Respondent’s Primary Supplier for Instruments for Standard Real-Time PCR by Market Segment

Table 24: Market Share Leaders for Standard & RT-PCR Instruments

Table 25: Percentage of Respondents Satisfied with Various Nucleic Acid Amplification Product Categories and Reasons for Interest in Switching

Table 26: Percentage of Respondents Satisfied with Nucleic Acid Amplification Product Categories - Comparison to 2007 Nucleic Acid Amplification Dashboard

Table 27: Most Important Features of Products for Nucleic Acid Amplification - Comparison to 2007 Nucleic Acid Amplification Dashboard

Table 28: Respondent’s Primary & Secondary Applications for End Products from Standard PCR with Taq Polymerase - Comparison to 2007 Nucleic Acid Amplification Dashboard

Table 29: Respondent’s Primary & Secondary Applications for End Products from Standard PCR with a Proofreading (High Fidelity) Polymerase - Comparison to 2007 Nucleic Acid Amplification Dashboard

Table 30: Respondent’s Primary & Secondary Applications for End Products from qPCR (genomic DNA template) using Taq Polymerase-Comparison to 2007 Nucleic Acid Amplification Dashboard

Table 31: Respondent’s Primary & Secondary Applications for End Products from qRT-PCR (cDNA template) using Taq Polymerase- Comparison to 2007 Nucleic Acid Amplification Dashboard



Abstract

Nucleic acid amplification is one of the most commonly performed molecular biology techniques and is a necessary precursor to a range of methods from gene cloning and site-directed mutagenesis to the quantitative analysis of gene expression. The Nucleic Acid Amplification Dashboard was developed from responses to a 21-question survey by 485 scientists predominantly located in North America and Europe. This Dashboard reveals key market indicators for the nucleic acid amplification market as a whole as well as for the following sub-segments:
  • Standard PCR with Taq polymerase, hot start Taq polymerase and proofreading polymerases
  • qPCR with Taq polymerase and with hot start Taq polymerase
  • qRT-PCR with Taq polymerase and with hot start Taq polymerase
  • Instruments for Standard and Real Time PCR"
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