Biopharmaceutical Expression Systems and Genetic Engineering Technology

Published by: BioPlan Associates, Inc.

Published: Nov. 1, 2008 - 415 Pages

Table of Contents

Cell-free systems

Cell-free expression, ATP regeneration system

Cell-Free Protein Synthesis (Cell-Free)

Large ribosomal subunit proteins, E. coli - cell-free systems

Broad, cross- or multi-platform technologies

In vivo linearization; InVoLin; Meganuclease Recombination System (MRS) - improved transformation

Vectors, site-specific recombinase assembly - eukaryotes

Glycosylation, mammalian [Generic entry]

Aminoglycoside phosphotransferase marker; Neomycin phosphotransferase I (nptI) marker;

Geneticin (G-418) selection - universal

Cellulose binding domain (CBD) fusion protein affinity tags; pET-CBD - universal

Controllable Self-Cleaving Intein Derivative; IMPACT-CN; pH-sensitive self-cleaving fusion

protein affinity purification tag - universal

Cotransformation, Columbia University - eukaryotes, selection

Cre-lox mediated in vitro recombination; Cyclization Recombination/locus of X-over P1;

site-specific recombination (SSR) - universal; genetic recombination

deltaPhase; Elastin-like polypeptide (ELP) fusion protein tags - chromatography-free

purification; universal

Directed Nuclease Editor (DNE); Meganuclease Design - universal

Dual expression vectors; Dual Affinity ReTargeting (DART) - antibodies; bacteria and mammalian cells

Expression enhancers, oligonucleotides - universal

Expression factory; baculoworkstation - automated parallel expression

Fusion protein expression systems; Affinity purification tags and chaperones - universal

Fusion proteins, self-cleaving, pH-sensitive - universal

Glutathione-S-transferase (GST) fusion protein affinity tags; pGEX vectors

GUS reporter system (beta-glucuronidase); GUS control vector - prokaryotes; plant cells;mammalian cells, non-vertebrate

Heat shock Promoters (HSPs); HSP chaperones

His tags; Poly-Histidine fusion affinity tag technology; 6xHistidine-tag - univeral;purification tags

His-tags; Poly-Histidine fusion affinity tag technology - univeral; purification tags

Hybridomas (Kšhler and Milstein) - monoclonal antibodies

Biopharmaceutical Expression Systems and Genetic Engineering Technologies

Lambda recombination protein; Homologous recombination

Meganuclease Recombination System (MRS)/I-SceI; homologous recombination -

universal genetic recombination

Mucin promoters; IIM14 and IIM22 - vector enhancers

New Cabilly; Cabilly-Boss; Monoclonal antibody 2-chains expression - universal

phCMV vectors; GenePORTER - universal

Poly(A) polymerase

Promoters

Recombinant DNA; Protein Expression; Cohen-Boyer

Reconstituting chemically orthogonal directed engineering (ReCODE) -

Unnatural amino acids; UAAs; E. coli; yeasts; mammalian cells

Selectable markers; Selection of transformed cells; Reporter genes

Small Ubiquitin-like Modifier (SUMO) fusion protein tags; Split SUMOpro System;

Ubl-specific protease - universal

Strep-tag affinity fusion protein tag; Strep-Tactin purification - universal

Super core promoters; SCP1; Core promoters - “the strongest [promoters] ever made”

TAGZyme; dipeptidyl aminopeptidase I (DPPI; DAPase Enzyme; cathepsin C) -

cleavage of His tags; universal

TEV Protease; Tobacco Etch Virus (TEV) NIa protease - cleavage of fusion

protein tags; universal

Transfection - universal

Translation Engineering expression; CODA; Computationally Optimized DNA

Assembly; Hot Rod Genes; Controlled Ribosomal Pausing - universal

White collar complex (WCC) promoter; UV light induction - universal

Minos transposon cell transformation - insect larvae; also eukaryotes

Coconut Express cell-free translation - plants

Prokayotes

Bacterial cell expression technology (BCE); araB promoter - antibody fragments;E. coli; prokaryotes

Subtilisin (psub) fusion protein tag expression; Profinity eXact expression - E. coli;bacteria; yeasts; CHO cells

Tetracycline-induced expression repression - prokaryotes

Bacteria

Bacillus megaterium expression - E. coli alternative

Bacillus subtilis, super-oxidizing strains; Thiol-disulfide oxidoreductases (TDORs);

Thioredoxin A (TrxA) depletion - disulfide bridge optimization

Bacterial artificial chromosome (BAC) expression; pBAC vectors - bacteria

Caulobacter crescentus expression; PurePro Caulobacter Expression System -

Caulobacter bacteria hosts

Clostridium Expression System; NTNH promoter from Clostridium botulinum

Flavobacterium heparinum expression - glycoproteins

Lactococcus lactis htrA- expression - protease depletion

Lactose (lac) promoters; Isopropyl-beta-galactosidase (IPTG) induction - bacteria

NIsin-Controlled gene Expression (NICE); Lactococcus lactis expression;nisA promoter - antibiotic selection

P170 Expression System;Lactococcus lactis expression

Pfenex Expression; Pseudomonas fluorescens biovar I (MB101) - E. coli alternative

Plasmids stabilization

Pseudoalteromonas haloplanktis TAC125 (PhTAC125) - cold expression

Quasi-synthetic vectors; Synthetic gene sequences in vectors - bacteria

Ralstonia eutropha expression; Alcaligenes eutrophus expression

Rhodospirillum rubrum (bacterial) expression - membrane proteins

Staphylococcus carnosus expresion

Subtilin Regulated Gene Expression; SURE competency - B. subtilis

E. coli expression/vectors

CASCADE expression; pALEX1 plasmids - E. coli

Choline-binding fusion affinity tags - bacteria

Clean Genome E. coli; Stripped-down E. coli

GroEL,GroES chaperones; Chaperonins - proper folding; universal; E. coli

Methylobacterium extorquens (bacterium) expression

CANGENUS; Streptomyces (lividans and griseus) expression

Saccharomyces cerevisiae expression

Streptomyces stationary phase expression; SPE system; Streptomyces vectors;

Secreted Protease Production (SPP) System

Streptomycetes hyper-inducible expression; PnitA-NitR system; Caprolactam

induction - Streptomycetes

Yeasts

ApoLife Yeast Expression; S. cerevisiae Twin Cassette Plasmids - antibodies in yeast

Arxula adeninivorans expression - alternative yeast

Chrysosporium lucknowense expression; C1 Express Hyperproducing Protein

Expression System - fungi

CoMed system; Universal Yeast vectors; pCoMed vectors; Arxula

adeninivorans-derived TEF1 promoter

Fungal expression systems

GlycoFi technology; Next Generation Biotherapeutics - Pichia pastoris; yeasts;

glycosylation; antibodies

Hansenula expression (yeast)

Hansenula polymorpha (yeast) expression - E. coli alternative

Kluyveromyces lactis expression; pKLAC1; Acetamidase/Acetamide selection;

K. lactis GG799 - yeast

Entry Number Monograph Titles

Biopharmaceutical Expression Systems and Genetic Engineering Technologies

xiv

NeuBIOS expression; Neurospora crassa expression; NeuKARYON - filamentous fungi;glycosylation; Cabilly-Boss workaround

Neurospora expression; cotA promoter - fungi

Ophiostoma expression - ascomycetes fungi

Yeast expression systems

Zygosaccharomyces bailii expression; Zbleu2 strain - yeast

EASYEAST; Saccharomyces cerevisiae strains - easy protein release

Yeast cell lines and vectors; Saccharomyces cerevisiae cell lines - proper folding and glycosylation

Pichia pastoris expression

VelociMab; EESYR expression system; FASTR cell lines - CHO expression optimization;antibodies

Plants

Plastid Transformation; Translation-based vectors (TBV) - plants

Chlamydomonas reinhardtii chloroplast expression; promoter - algae, single-cell

Chlamydomonas reinhardtii expression - algae, single-cell

Drosophila Expression System (DES) - insect cell culture

Streptomyces lividans

Eukaryotes

Antibiotic inducible promoters - eukaryotes

AttSite recombinases - precise gene insertion; eukaryotes

Bovine growth hormone (bGH) polyadenylation sequence - eukaryotes; expression enhancement

Expression enhancers; Copy number increase - eukaryotic cells

Homologous Recombination; Knock-out/knock-in animals and cells

Internal Ribosome Entry Sequences (IRES) RNA translation enhancers; pCITE vectors;

Cardiovirus 2A IRES; pIRES - eukaryotes

Light-switchable promoters

Tetracycline (Tc; Tet) Expression Systems - eukaryotes

Zinc finger DNA-binding proteins (ZFPs); ZFP Transcription Factors - gene modification; mammalian cells; plant cells

Protozoa

Ciliate Performance Expression System; CIPEX system;

Tetrahymena (protozoa) expression

LEXSY; Leishmania tarentolae expression system - protozoa

Perkinsus marinus expression - protozoa express large proteins

TetraExpress; Tetrahymena (protozoan) expression

Tetrahymena thermophila (protozoan) expression

Animals, misc.

Milk protein promoter - proteins in milk of transgenic animals

Shrimp expression; Penaeus stylirostris expression; Taura Syndrome Virus (TSV)

IRES vectors - glycoproteins; antibodies

Transgenic rabbits - humanized polyclonal antibodies

Mammalian

AmProtein vectors - “stongest mammalian vector set”; universal

Anti-apoptosis expression system; BCL-xL or BCL-2 expressing cell lines -

CHO, NS0, BHK, SP2/0-Ag14

Autocatalytic cleavage sites; Mengo virus vectors; Scission cassettes - mammalian cells

Cell adhesion optimization; cdkl3, siat7e, and lama4 genes - antibody-expressing cells

CMV (human) promoter; Cytomegalovirus promoter; Complete Control Inducible

Mammalian Expression System - mammalian cells

Cumate gene-switch; Q-mate Inducible Expression - mammalian cells

Dihydrofolate reductase (DHFR) System - selectable marker/amplification;

CHO and NS0 cells

Flp-In expression system; FLP-Mediated Gene Modification in Mammalian Cells; FLP

recombinase - mammalian cells

Gene-Activated (GA) expression, in vivo - mammalian cells

Glutamine synthetase (GS) System - NS0, CHO, mammalian cells

GPEx Gene Product Expression Technology - mammalian; CHO

MARtech; Matrix Attachment Regions; Scaffold Attachment Regions;

SARs - mammalian cells

RheoSwitch Mammalian Inducible Expression System; RheoSwitch Ligand RSL1

promoter; Ecdysone receptor induction - mammalian cells; adjustable expression

Selexis Genetic Elements (SGEs) - mammalian cell lines

STabilizing Anti-Repression; STAR elements - expression enhancement; mamalian cells

Ubiquitous chromatin opening element (UCOE) expression - mammalian cells

Whey acidic protein (WAP) milk promoters - mammals

Chinese hamster ovary (CHO) cells

ACE Expression System - MAb-expressing CHO cells lines

Boehringer Ingelheim High Expression System (BI HEX); CHO-DG44

CHO cell line (Puck); CHO-K1 cell line

CHO DG44 cells, DHFR-; CHO-DG44; DUK-XB11; CHO K1 DUX B11 (DHFR-) cells;

Dihydrofolate reductase selection/amplification, CHO cells

CHO SSF cell lines - adherent CHO cells; protein-free media

CHO Supercell; Targeted transfection; CHO DG44 cell line

CHOZn CHO DG44 cell lines - antibodies

GS-CHO Protein Free System; CHOK1SV cell lines - protein-free media

Sympress expression; Human polyclonal antibodies (rpAB) - CHO cells

Baby hamster kidney (BHK) 21 cells; ATCC CCL 10

Hybridomas

Human MORPHODOMA; Morphogenics; Hypermutation; PMS2 gene screening,

modification - human monoclonal antibodies from hybridomas

Cell-free system - glycoproteins; hybridoma

Ex-Cell EBx expression; EBx cells; Chicken embryonic stem cells;

Chicken EBx cells; Duck EBx cells

Chickens/Poultry

Chicken egg expression; Transgenic animals

Chicken rimordial germ cells (PGCs) - Human proteins/monoclonal antibodies in chicken eggs

OVA System; Avian Transgenic Biomanufacturing - chicken eggs expresssion

Transgenic poultry; avian transgenesis and nuclear transfer - proteins in chicken eggs

Windowing Technology - transgenics avians/chickens

Human

CEVEC Amniocyte Production (CAP) expression; Human amniocytes, immortalized

bcl-2 (p21) overexpressing NS0 cell lines - NS06A1(100)3 cell line - antagonize

apoptosis; Mabs

Cell fusion, NS0 cells - antibodies

Cholesterol/3-ketosteroid reductase (p3-KSR) expression - cholesterol

selection/induction of NS0 cells

GlycoExpress human cell lines; NM-F9 cell line - glycolysis

Human primary preB lymphocytes; HupreB cells - human monoclonal antibodies

NS0 murine myeloma cell line

NS0-PFCF cells; NS0 cells, protein- and cholesterol-free - antibodies

PER.C6 expression; Extreme density (XD) Technology - glycoproteins; antibodies

Retrotransposon vectors - transgenic avian/chicken cell culture

Sp2/0-Ag14 cells - protein-free media; antibodies

HEK 293 cell line, protein- and peptide-free (Hektor G) media; human

embryonic kidney (HEK 293) cell line -

HEK 293 expression

HEK 293 expression

HEK293 cell lines

HEK293SFE cell line

HKB-11 (HKB11) expression; Hybrid of kidney and B cells - HEK-293 alternative

Plants

Agrobacterium tumefaciens; Ti plasmids - transgenic plants

Antibiotic resistance markers - plants

Chloroplast expression - plants

Chloroplast Transformation Technology (CTT) - plant cells

Coupled regeneration/ transformation, plants

GENEWARE expression; Tobacco mosaic virus (TMV) vectors - tobacco; plants

Glyco-Engineered Moss; Physcomitrella patens expression; moss expression

promoting regions (MEPRs) - glycosylation; antibodies

iBioLaunch expression; Launch vectors - proteins and Mabs in plants

LEX System; Lemna (duckweed) expression - algae, whole plants

Nuclear transfer Cultured inner cell mass cells (CICM) - transgenic animals;

cloning from somatic cells

Nuclear Transformation Suite, plants

Plant expression - glycosylation

pPIPRA vectors - plants; public domain

ProCellEx Plant Expression - plant cells; glycosylation

Proficia expression - transient expression, plants

RNA-dependent RNA polymerase (RdRP) - universal

Stratosome Biologics System; Oilbody expression; Safflower plant seed expression

Super-mas Plant Gene Promoter; Gelvin promoter; (Aocs)3AmasPmas - plants

TransBacter Gene Transfer System -plants; royalty-free

Ubiquitin linkage domain - multiple proteins in transgenic plants

Ubiquitin plant promoters

Zara technology; Protein body-inducing sequence (PBIS) fusion proteins;

Recombinant protein body-like assembly (RPBLA); StorPro organelles

(protein encapsulation); Prolamin fusion proteins - eukaryotes

CleanGene plant transformation

Plastids (chloroplasts) expression - plant cell culture

Insects

High Five cell line (BTI-TN-5B1-4, ATCC CRL 10859); Trichopulsia ni cell line -baculovirus host cells; insect cell culture

Insect cells glycosylation

Insect cells glycosylation

Insect cells/Baculovirus expression systems; Baculovirus expression

vector systems (BEVS)

PERLXpress; TRANSPILLAR larvae; Trichoplusia ni larvae expression -

transformed caterpillars

Trichoplusia ni (cabbage looper) cell lines; BTI-TN-MG1; ATCC CRL 10860;

BTI-TN-5B1-4; ATCC CRL 10859

Trichoplusia ni (cabbage looper) cell lines; H5CL-B and H5CL-F;

BTI-TN-5B1-4-derived insect cell lines

Baculovirus expression vector systems (BEVS)

Entry Number Monograph Titles

InsectSelect Protein Expression System - insect cells; avoid baculoviruses

Mimic Sf9 Insect Cells - mammalian-like glycosylation

Polydnavirus vectors - insect cells; baculovirus altnerative

Pre-occluded Virus (POV) baculovirus vectors; Insect cells, per os (oral)

baculovirus infection

Spodoptera frugiperda Sf-21 cell line - baculovirus host insect cells

Spodoptera frugiperda Sf-9 cell line - baculovirus host insect cells

NusA E. coli fusion proteins - eukaryotes; protein solubilization

Other broad/universal and older technologies

Enterokinase - fusion protein cleavage

Phage lambda promoters; PL promoter; PR promoter - E. coli

Phage T5 promoter

WI-38 cell line, Normal human fetal lung fibroblasts

Alkaline Phosphatase; Calf Intestinal Alkaline Phosphatase (CIAP) - prevent vector recircularization

Benzonase; Serratia marcescens endonuclease - polynucleotides breakdown

DNA Ligase (E. coli)

Site-directed mutagenesis

T4 DNA Ligase

T4 RNA Ligase

More Specific and Component Technologies

Altogen transfection; RNAi gene silencing

Bacteria/Prokaryotes

Profuse vectors; Cisperone chaperone fusion tags - E. coli; Saccharomyces cerevisiae

Bacterial transcription promoters

BresaGen fusion protein expression - E. coli

Cold-Induced expression - Bacillus subtilis

desA promoter, iron-regulated; DmdR repressors - Actinomycetes

E. coli. vectors; Mnt-Arc promoters; T1 and T2 rrnB ribosomal terminators - bacteria

pAVEway expression - E. coli and Pseudomonas

pTAT-HA plasmids - E. coli; bacteria

Twin-arginine translocation (Tat) system; Tat nanomachine - protein folding, then secretion;bacteria

VegI promoters - Bacillus subtilis and E. coli

Xer-cise gene excision; Xer recombinases - Bacillus subtilis

Agrobacterium tumefaciens RpoA co-expression transcriptional activator - E. coli

Avidin affinity fusion protein affinity tags; Biotin purification; PinPoint Xa

Protein Purification System - E. coli

Biogenerics manufacturing technology packages - E. coli

BL21(DE3) competent E. coli cells

C-LYTAG affinity fusion protein tag; Streptococcus pneumoniae

N-acetylmuramoyl-L-alanine amidase LytA - E. coli; purification

Campylobacter jejuni glycosylation genes; OTase of C. jejuni - glycosylation; E. coli

Chaperone expression plasmids; DnaK, DnaJ and GrpE chaperones - E. coli

cis-Acting Peptide chaperones - E. coli

Codon-Optimized, Expression-Ready E. coli Clones

Continuous culture - bacteria; E. coli

Disulfide isomerase coexpression; DsbC and DsbG - E. coli; disulfide bonds and folding

Elastin-like polypeptide (ELP) self-cleaving fusion protein tags - chromatography-free purification; E. coli

ExpressProtect; p26, SicA, and alpha-crystallin-type fusion protein tags - E. coli

High copy number plasmids; pBGP120 - E. coli

High transformation efficiency (Hte) competency - E. coli cells

His-Patch ThioFusion System; pThioHis vector - E. coli; fusion proteins

N(pro) fusion protein tag, self-cleaving; Swine fever virus N(pro) autoproteolysis;

EDDIE - E. coli

OverExpress C41(DE3) and C43(DE3) - E. coli strains

OxlT plasmids; Oxalate/Formate Exchange Protein - E. coli

pBR322 - E. coli plasmid; antibiotic selection

pCold vectors; Cold Shock Protein A (cspA) promoters - E. coli, cold induction

pET Expression System; T7 promoter; pET Directional TOPO Cloning; Champion pET

Expression Vectors; T7 RNA polymerase (T7 RNAP) - E. coli

pMAL Protein Fusion and Purification System; Maltose binding (MBP) fusion

affinity tags; pMAL plasmids - antibody fragments; E. coli

Polyhydroxybutyrate (PHB) fusion tags, self-cleaving coexpressed with

affinity medium - E. coli; universal

Red/ET recombination; ET cloning/ET recombination; GET recombination;

Recombineering; £f Red-mediated recombination; lambda-mediated

recombination - E. coli

Skp and DsbC chaperone fusions - E. coli; secretion control

Tac promoters - E. coli

Tryptophan (trp) promoters - E. coli

WACKER Secretion System; E. coli K12-based secretion system - antibody fragments

Flavivirus vectors; Kunjin replicon vectors - prokaryotes; Streptomyces; prokaryotes

Streptomyces inducers

Streptomyces lividans strains; xysA promoters

Yeasts

AlcoFree Yeasts; Saccharomyces cerevisiae KOY.TM6* strains

ALEU2 marker; AHSB4 promoter; Arxula adeninivorans expression

Aspergillus niger expression; A. niger A4 promoters - humanized antibodies

Aureobasidin A vectors (pAUR) - selectable marker in yeasts

Calnexin chaperone - Hansenula polymorpha; yeasts

Chitin synthase (CHS1), Yeast growth factor, chitin synthase (CHS1) -

yeast promoter discovery

Estradiol-dependent enhancer; Gal4-ER-VP16 - yeasts

Formaldehyde dehydrogenase (FLD1) promoter; Formaldehyde selection -

Pichia pastoris; yeasts

GAPFL promoter; Glyceraldehyde-3-phosphate dehydrogenase-derived

promoter - yeasts

Gene Design, algorithmic - yeasts

Hypermutable yeast

PH05 promoter; Phosphate induction -yeasts

Vesicular fusion factor 2 protein (Vff2p) enhancer - yeast; bacteria; CHO cells

Vesicular fusion factor 2 protein (Vff2p) enhancer - yeasts

Xplor Vector System, yeast optimization

Antibody fragment expression - Saccharomyces cerevisiae

Saccharomyces cerevisiae, cold induction

Secretion Enhancer Vector System (SEVS); SecHancer vector - Saccharomyces cerevisiae

Mab Xpress Antibody Production System; Pichia pastoris - glycosylated antibodies

Pichia expression, rhamnose induction

Pichia GlycoSwitch System; Glycoswitch plamids - yeasts; glycosylation

Pichia pastoris antibody expression

Pichia pastoris AOX1 promoters

Pichia pastoris “super yeast”; Pichia pastoris AOX1 promoters

Aminoglycoside adenylyltransferase (aadA1) promoter - bacteria; eukaryotes

ColE1 plasmids, E. coli - prolonged viability

Mammals

CMV/R Promoter - eukaryotes

piggyBac transposon - eukaryotes; insect cells

Tax-inducible expression; Bovine leukemia virus (BLV) promoter - mammalian cells

BacMam; pBacMam vectors - mammalian vectors, baculovirus-based

Calnexin, calreticulin, Erp57, Hsp40, and Hsp70 chaperones - mammalian cells

CCT promoters - mammalian cells

ClonePixFL Selection - antibodies, mammalian cells

Hsp60, Hsp70, Hsp90, Hsp100 chaperones - mammalian cells

IRF-1 estrogen receptor promoter; Estradiol induction - mammalian cells

Osteoclast-associated receptor (OSCAR) promoter - mammalian; CHO cells

pAccAB vectors - antibodies; mammalian cells

RP Shift; Senescence induction; PACE Expression Vector - mammalian

cells; expression enhancement; antibodies

Semliki forest virus (SFV) vectors - mammalian and insect cells

Chinese Hamster Ovary (CHO) cells

CHEFl expression; CHO elongation factor-la (EF-Ialpha) promoter - CHO cells

CHO cells - antibodies; serum-free media

CHO cells dhfr RNA interference; RNA silencing vectors - CHO cells

CSL4S-342 CHO cells (CHO-K1 CSL4S-342)

Osteoclast-associated receptor (OSCAR) promoter - mammalian; CHO cells

Pangen CHO expression

StableFast Biomanufacturing System; pBFdfhr.2 Expression Vector -

CHO cells; antibodies

Tandem Chimeric Antibody Expression (TCAE) vectors; ANEX vectors;

CHO cell line TCAE 8 (ATCC 9119); Kozak sequences, impaired - antibodies

UTR Clone Generation; UTRtech; “Cell Factories”; Gaussia luciferase signal

peptides - Mabs supersecretion; CHO cells

DNA microinjection - transgenic animals, chickens

HEK-293 cells

293ST-3F6 cell line; HEK-293 adapted to SFM

CRE-inducible expression; cyclic AMP response elements (CREs) - HEK-293 cells

HEK-293 expression

HEK-293 expression

pTT vectors for HEK-293E cells

Plants

Magnifection; magnICON; Transgene Operating System (TOS) - antibodies;

plants and plant cells

MARs PLUS; Matrix attachment regions PLUS - plants

Concert Plant-Cell-Produced system - tobacco plant cell culture

Phyton plant cell fermentation

ExpressTec expression; ExpressPro; ExpressMab - rice and barley; antibodies

Insects

AcMNPV p35 apoptosis inhibition; Sf9P35AcV5-1 and Sf9P35AcV5-3 -

insect cells, apoptosis resistance

AF 99 insect cell line

BL-Sf-21AE-Cl 3 cell line - Insect cell lines, baculovirus hosts

Cre/loxP Recombination-Mediated Cassette Exchange (Cre/loxP RMCE) - Drosophila

(mosquitos)

Drosophila expression

Drosophila melanogaster S2 cells; Drosophila-SFM.D.Mel-2 Cells; Schneider S2

Drosophila cells; S2 cells, SFM - insect cell culture

IE-1 (BmNPV 1.2 kb fragment) promoters; Bombyx mori actin promoters - insect cells

Insect cell lines - baculovirus hosts

Insect cells, per os (oral) baculovirus infection

Lymantria diapar nucleopolyhedrovirus and L. dispar 652Y (Ld652Y) cell lines -

baculovirus host cells

pIEx baculovirus vectors; hr5 enhancer; ie1 immediate early promoter - insect cells;

avoid baculovirus pathogenicity

Rhopalosiphum padi virus Internal Ribosome Entry Sequence (IRES);

Picorna-like virus IRES; Drosophila IRES - insect cells; plant cells

Tni PRO; Trichoplusia ni cell line

BAC-TO-BAC Baculovirus Expression System; baculovirus shuttle vectors;

bacmids - insect vectors produced in E. coli

BacTen System; p10 promoter vectors - insect cells

Baculovirus vectors and promoters - glycosylation

BestBac vectors; Autographa californica nuclear polyhedrosis virus (AcNPV) vectors -

insect cells

Drosophila sialyltransferases - insect cells; glycosylation

Sapphire baculovirus expression - disulfide bond formation

Vankyrin enhanced baculovirus expression vector system (VE-BEVS);

Vankyrin-enhanced cell lines (VE-CL)

Indexes

Company/Organization Index

Subject Index

Primary Host/Organism Index

Abstract

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