Next-Generation Sequencing: Scientific and Commercial Implications of the $1000 Genome

Published by: CHI Insight Pharma Reports

Published: Aug. 1, 2007 - 88 Pages

Table of Contents

SECTION 1

A BRIEF HISTORY OF GENOME SEQUENCING TECHNOLOGY AND MILESTONES

Dideoxy (Sanger) Sequencing

Automated DNA Sequencing

Genome Wars

The $1,000 Genome

SECTION 2

BEYOND SANGER SEQUENCING: NEXT-GENERATION SEQUENCING TECHNOLOGIES

Sequencing-by-Synthesis/Pyrosequencing

Sequencing-by-Synthesis

Illumina

GE Healthcare

Intelligent Bio-Systems

SOLiD (Supported Oligonucleotide Detection)

Storage

Polony Sequencing

Single-Molecule Sequencing

VisiGen

Pacific Biosciences

Nanopore Sequencing

Optical Trapping

Nanoscale Sanger Sequencing

SECTION 3

APPLICATIONS OF NEXT-GENERATION SEQUENCING

The $1,000 Genome/Personal Genomics

The Cancer Genome Atlas

Evolutionary Genomics

Environmental Genomics

Gene Regulation

SECTION 4

EXPERT INTERVIEWS WITH TECHNOLOGY INNOVATORS

Tony Smith, PhD, Illumina

Susan Hardin, PhD, VisiGen

Jay Shendure, MD, PhD, University of Washington, Seattle

Kevin McKernan, ABI

Stanley N. Lapidus, Helicos

SECTION 5

NEXT-GENERATION COMPANIES

Applied Biosystems

GE Healthcare

Helicos BioSciences

Illumina/Solexa

Intelligent Bio-Systems

Pacific Biosciences

Reveo

Roche/454 Life Sciences

VisiGen

SECTION 6

CHI INSIGHT PHARMA REPORTS—NEXT-GENERATION SEQUENCING SURVEY—FEBRUARY 2007

APPENDIX

NHGRI RESEARCH GRANTS

$1,000 Genome Grants (August 2005)

$100,000 Genome Grants

References

Company Index with Web Addresses

Abstract

A growing number of companies and academic groups are reporting impressive progress in commercializing new sequencing platforms that offer orders of magnitude of improved throughput and cost. The prospect of routine, personalized genome sequencing is suddenly within reach, not to mention spectacular advances in a host of related fields. This new report from CHI Insight Pharma Reports describes and assesses these developing technologies and their applications:

• Pyrosequencing
• Sequencing by synthesis
• Supported oligonucleotide detection (SOLiD)
• Single-molecule sequencing
• Nanopore sequencing
• Optical trapping

Next-Generation Sequencing: Scientific and Commercial Implications of the $1000 Genome also includes the results and analysis of a quantitative Web survey of the views, practices, and plans of individuals at life science organizations involved with gene sequencing.

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