Next Generation Sequencing Sample Preparation Market Size, Share, Trends, Industry Analysis Report By Product & Service (Instruments, Consumables, Services), By Workflow, By Application, By End Use, By Region – Market Forecast, 2025–2034

The next generation sequencing sample preparation market size is expected to reach USD 16.57 billion by 2034, according to a new study by Polaris Market Research. The report “Next Generation Sequencing Sample Preparation Market Share, Size, Trends, Industry Analysis Report By Product & Service (Instruments, Consumables, Services), By Workflow, By Application, By End Use, By Region; Market Forecast, 2025–2034” gives a detailed insight into current market dynamics and provides analysis on future market growth.

Next generation sequencing (NGS) sample preparation is a critical step in the sequencing workflow that involves processing DNA or RNA samples to make them compatible with high-throughput sequencing platforms. The preparation usually begins with nucleic acid extraction, where DNA or RNA is isolated from biological samples such as blood, tissue, or cells. The extracted material is then quantified and assessed for purity using spectrophotometry or fluorometry. For RNA sequencing (RNA-seq), an additional step of converting RNA into complementary DNA (cDNA) is required. The DNA or cDNA is then fragmented into smaller pieces, either enzymatically or mechanically, to facilitate sequencing.

Next generation sequencing sample preparation is used across various applications, including whole genome sequencing, targeted DNA RNA sequencing, transcriptomics, epigenetics, and metagenomics. In clinical diagnostics, it enables the identification of genetic mutations linked to diseases, while in cancer research, it helps characterize tumor genomes. In infectious disease studies, NGS aids in pathogen detection and outbreak surveillance.

Advancements in library preparation kits and automated systems have streamlined the process, reducing hands-on time and improving reproducibility. NGS sample preparation is the foundation for generating high-quality sequencing data, enabling breakthroughs in genomics, personalized medicine, and biotechnology.

Next Generation Sequencing Sample Preparation Market Report Highlights

In terms of product & service, the consumables segment accounted for a major market share in 2024. Researchers and laboratories across clinical, academic, and pharmaceutical sectors heavily relied on reagents, kits, and other disposable materials due to the high frequency of sample preparation workflows.

Based on workflow, the library preparation & amplification segment held the largest share of the next generation sequencing (NGS) sample preparation market in 2024 due to its crucial role in enabling accurate and high-throughput sequencing.

In terms of application, the oncology segment held the largest market share in 2024 due to the ability of next generation sequencing sample preparation to uncover somatic mutations, identify tumor-specific biomarkers, and guide targeted therapies.

Based on end use, the pharma & biotech entities segment accounted for a major market share in 2024. These organizations drove substantial demand for sequencing sample preparation due to their extensive use of NGS technologies in drug discovery, biomarker identification, and companion diagnostics.

A few global key players in the NGS sample preparation market include Agilent Technologies, Inc.; BGI Genomics; Bio-Rad Laboratories, Inc.; Danaher Corporation; Eurofins Scientific; F. Hoffmann-La Roche Ltd; Illumina, Inc.; PerkinElmer Inc.; QIAGEN; and Thermo Fisher Scientific Inc.

Polaris Market Research has segmented the next generation sequencing sample preparation market report on the basis of product & service, workflow, application, end use, and region:

By Product & Service Outlook (Revenue, USD Billion, 2020–2034)

Instruments

Consumables

Services

By Workflow Outlook (Revenue, USD Billion, 2020–2034)

Nucleic Acid Extraction

Library Preparation & Amplification

Target Enrichment

Library Quantification/QC

By Application Outlook (Revenue, USD Billion, 2020–2034)

Oncology

Clinical Investigation

Reproductive Health

HLA Typing/Immune System Monitoring

Metagenomics, Epidemiology & Drug Development

Agrigenomics & Forensics

Consumer Genomics

By End Use Outlook (Revenue, USD Billion, 2020–2034)

Academic Research

Clinical Research

Hospitals & Clinics

Pharma & Biotech Entities

Other

By Regional Outlook (Revenue, USD Billion, 2020–2034)

North America

US

Canada

Europe

Germany

France

UK

Italy

Spain

Netherlands

Russia

Rest of Europe

Asia Pacific

China

Japan

India

Malaysia

South Korea

Indonesia

Australia

Vietnam

Rest of Asia Pacific

Middle East & Africa

Saudi Arabia

UAE

Israel

South Africa

Rest of Middle East & Africa

Latin America

Mexico

Brazil

Argentina

Rest of Latin America Show more