U.S. Next-Generation Sequencing Library Preparation Market Size, Share, Trends, Industry Analysis Report By Sequencing Type (Targeted Genome Sequencing, Whole Genome Sequencing), By Product, By Application, By End Use – Market Forecast, 2025–2034

The U.S. next-generation sequencing library preparation market size is expected to reach USD 2,167.71 million by 2034, according to a new study by Polaris Market Research. The report “U.S. Next-Generation Sequencing Library Preparation Market Share, Size, Trends, Industry Analysis Report By Sequencing Type (Targeted Genome Sequencing, Whole Genome Sequencing), By Product, By Application, By End Use; Market Forecast, 2025–2034” gives a detailed insight into current market dynamics and provides analysis on future market growth.

Next-generation sequencing library preparation is a crucial step in the sequencing workflow, involving the conversion of DNA or RNA samples into a format compatible with high-throughput sequencing platforms. The process includes fragmenting the nucleic acids, attaching specialized adapter sequences, and amplifying the material to generate a sequencing-ready library. The quality of library preparation directly impacts the accuracy and efficiency of sequencing.

Next-generation sequencing library preparation is widely used across genomics research, clinical diagnostics, and biotechnology. In whole-genome sequencing (WGS), it enables comprehensive analysis of an organism’s DNA, aiding in genetic variant detection and evolutionary studies.

U.S. Next-Generation Sequencing Library Preparation Market Report Highlights

In terms of sequencing type, the targeted genome sequencing segment accounted for 63.37% of revenue market share in 2024. Clinical researchers and diagnostic laboratories favored this approach due to its cost-effectiveness, high accuracy, and efficiency in identifying specific genetic variants associated with inherited diseases and cancers.

The whole genome sequencing segment, based on sequencing type, is projected to hold a substantial share in the coming years, owing to advancements in sequencing technologies and declining costs.

Based on product, the reagents & consumables segment dominated the market share in 2024 due to recurring demand for library preparation kits, enzymes, adapters, and amplification reagents used in every sequencing run.

In terms of application, the drug & biomarker discovery segment held the largest revenue share in 2024 due to the crucial role of next-generation sequencing library preparation in precision medicine and pharmaceutical research.

Based on end use, the academic and research institutions segment accounted for 37.83% of revenue market share in 2024 due to strong federal and private funding for genomics research and large-scale sequencing projects.

A few key players in the U.S. next-generation sequencing library preparation market include Agilent Technologies, Inc.; BD; F. Hoffmann-La Roche AG, Inc.; Illumina, Inc.; New England Biolabs Inc.; Oxford Nanopore Technologies; Pacific Biosciences of California, Inc.; QIAGEN N.V.; Revvity, Inc.; Thermo Fisher Scientific Inc.; and Twist Bioscience.

Polaris Market Research has segmented the U.S. next-generation sequencing library preparation market report on the basis of sequencing type, product, application, and end use:

By Sequencing Type Outlook (Revenue, USD Million, 2020–2034)

Targeted Genome Sequencing

Whole Genome Sequencing

Whole Exome Sequencing

Other

By Product Outlook (Revenue, USD Million, 2020–2034)

Reagents & Consumables

DNA Library Preparation Kits

RNA Library Preparation Kits

Other

Instruments

By Application Outlook (Revenue, USD Million, 2020–2034)

Drug & Biomarker Discovery

Disease Diagnostics

Cancer Diagnostics

Reproductive Health Diagnostics

Infectious Disease Diagnostics

Other

Others

By End Use Outlook (Revenue, USD Million, 2020–2034)

Hospitals and Clinics

Academic and Research Institutions

Pharmaceutical and Biotechnology Companies

Others Show more