Western blotting is a laboratory technique used to detect a specific protein in a blood or tissue sample. The method involves using gel electrophoresis to separate the sample's proteins. The separated proteins are transferred out of the gel to the surface of a membrane. The membrane is exposed to an antibody specific to the target protein. The binding of the antibody is detected using a radioactive or chemical tag. A western blot is sometimes used to diagnose disease. By using a western blot, researchers can identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. Various steps in western blotting include tissue preparation, gel electrophoresis, transfer of proteins onto the membrane, blocking to prevent membrane and antibody interactions, incubation of membrane, and antibody-based detection of proteins.
Market Dynamics
Increasing adoption of organic growth strategies, such as increasing awareness about western blotting by key players in the market to expand their product portfolio, is expected to drive market growth over the forecast period. For instance, in January 2021, Bio-Rad Laboratories, Inc., a developer and manufacturer of specialized technological products for life science research and clinical diagnostics, announced the launch of the Bio-Rad Western Blotting Learning Center, an online library that provides comprehensive resources, information, and guidance to help researchers improve their experimental western blotting (or immunoblotting) approaches to obtain high-quality data.
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