Nucleic Acid Amplification Life Science Dashboard™ Series 1

Published by: Percepta Associates, Inc.

Published: Jan. 1, 2007 - 153 Pages

Table of Contents

Executive Summary

Key Findings and Implications

Nucleic Acid Amplification Dashboard

Nucleic Acid Amplification Market Opportunity Matrix

Survey Methodology

Survey Invitation Text

Respondent Demographics

Frequency of Performance of Molecular Biology Techniques

Frequency of Performance of Nucleic Acid Amplification

Reaction Throughput and Market Growth Rates

Respondent’s Stated Price Per Reaction

Total Market Size, Market Segment Sizes and Total Market Growth Rate

Market Shares for Products for Standard PCR

Market Shares for Products for qPCR with Genomic DNA Templates

Market Shares for Products for qRT-PCR with cDNA Templates

Market Shares for Instruments for Standard PCR and Real-Time PCR

Customer Satisfaction And Interest In Switching Suppliers

Product Features That Influence Purchasing Decisions

Primary and Secondary Downstream Applications

Desired Changes to Nucleic Acid Amplification Products

Survey Questionnaire

List of Figures and Tables

Figure 1: Respondent’s Place of Employment

Figure 2: Respondent’s Country/Region

Figure 3: Respondent’s Job Title

Figure 4: Respondent’s Areas of Expertise/Specialization

Figure 5: Number of Employees in Respondent’s Laboratories

Figure 6: Percentage of Respondents Performing Various Techniques at Least a Few Times per Year

Figure 7: Percentage of Respondents Performing Nucleic Acid Amplification

Figure 8: Percentage of Respondents Performing Various Nucleic Acid Amplification Techniques at Least a Few Times per Year

Figure 9: Percentage of Respondents Performing Standard PCR with Taq Polymerase

Figure 10: Percentage of Respondents Performing Standard PCR with a Hot Start Taq Polymerase

Figure 11: Percentage of Respondents Performing Standard PCR with a Proofreading (High Fidelity) Polymerase

Figure 12: Percentage of Respondents Performing qPCR (Genomic DNA Template) Using Taq Polymerase

Figure 13: Percentage of Respondents Performing qPCR (Genomic DNA Template) Using Hot Start Taq Polymerase

Figure 14: Percentage of Respondents Performing qRT-PCR (cDNA Template) Using Taq Polymerase

Figure 15: Percentage of Respondents Performing qRT-PCR (cDNA Template) Using Hot Start Taq Polymerase

Figure 16: Respondent’s Primary Supplier for Standard PCR with Taq Polymerase

Figure 17: Respondent’s Primary Supplier for Standard PCR with Taq Polymerase by Region

Figure 18: Respondent’s Primary Supplier for Standard PCR with Taq Polymerase by Organization Type

Figure 19: Respondent’s Primary Supplier for Standard PCR with a Hot Start Taq Polymerase

Figure 20: Respondent’s Primary Supplier for Standard PCR with a Hot Start Taq Polymerase by

Figure 21: Respondent’s Primary Supplier for Standard PCR with a Hot Start Taq Polymerase by Organization Type

Figure 22: Respondent’s Primary Supplier for Standard PCR with a Proofreading (High Fidelity) Polymerase

Figure 23: Respondent’s Primary Supplier for Standard PCR with a Proofreading (High Fidelity) Polymerase by Region

Figure 24: Respondent’s Primary Supplier for Standard PCR with a Proofreading (High Fidelity) Polymerase by Organization Type

Figure 25: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Taq Polymerase

Figure 26: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Taq Polymerase by Region

Figure 27: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Taq Polymerase by Organization Type

Figure 28: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Hot Start Taq Polymerase

Figure 29: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Hot Start Taq Polymerase by Region

Figure 30: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Hot Start Taq Polymerase by Organization Type

Figure 31: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Taq Polymerase

Figure 32: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Taq Polymerase by Region

Figure 33: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Taq Polymerase by Organization Type

Figure 34: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Hot Start Taq Polymerase

Figure 35: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Hot Start Taq Polymerase by Region

Figure 36: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Hot Start Taq Polymerase by Organization Type

Figure 37: Respondent’s Primary Supplier of Instruments for Standard PCR

Figure 38: Respondent’s Primary Supplier of Instruments for Standard PCR by Region

Figure 39: Respondent’s Primary Supplier of Instruments for Standard PCR by Organization Type

Figure 40: Respondent’s Primary Supplier of Instruments for Real-Time PCR Respondent’s

Figure 41: Respondent’s Primary Supplier of Instruments for Real-Time PCR by Region

Figure 42: Respondent’s Primary Supplier of Instruments for Real-Time PCR by Organization Type

Figure 43: Percentage of Respondents That Have Switched Suppliers in the Last Six Months

Figure 44: Most Important Features of Nucleic Acid Amplification Products

Figure 45: Respondent’s Primary Application for End Products from Standard PCR

Figure 46: Respondent’s Primary and Secondary Applications for End Products from Standard PCR

Figure 47: Respondent’s Primary Application for End Products from Standard PCR with a Proofreading Polymerase

Figure 48: Respondent’s Primary and Secondary Applications for End Products from Standard PCR with a Proofreading Polymerase

Figure 49: Respondent’s Primary Application for End Products from qPCR with a Genomic DNA Template

Figure 50: Respondent’s Primary and Secondary Applications for End Products from qPCR with a Genomic DNA Template

Figure 51: Respondent’s Primary Application for End Products from qRT-PCR with a cDNA Template

Figure 52: Respondent’s Primary and Secondary Applications for End Products from qRT-PCR with a cDNA Template

Table 1: Frequency of Performance of Various Molecular Biology Techniques

Table 2: Frequency of Co-Performance of Various Molecular Biology Techniques

Table 3: Frequency of Performance of Various Nucleic Acid Amplification Methods

Table 4: Frequency of Co-Performance of Molecular Biology Techniques with Nucleic Acid Amplification Methods

Table 5: Frequency of Co-Performance of Nucleic Acid Amplification Methods with Molecular Biology Techniques

Table 6: Median and Average Monthly Throughput for Nucleic Acid Amplification Methods

Table 7: Percentage of Respondents Performing Various Numbers of Amplification Reactions Per Month for Nucleic Acid Amplification Methods

Table 8: Projected Growth in the Performance of Various Nucleic Acid Amplification Methods

Table 9: Median and Average Price Per Prep for Nucleic Acid Amplification Products

Table 10: Market Share Leaders for Standard PCR Amplification Products

Table 11: Market Share Leaders for qPCR (Genomic DNA) Amplification Products

Table 12: Market Share Leaders for qRT-PCR (cDNA Template) Amplification Products

Table 13: Market Share Leaders for Instruments for Standard PCR and for Real-Time PCR

Table 14: Percentage of Respondents Satisfied with Various Nucleic Acid Amplification Techniques and Reasons for Dissatisfaction

Abstract

• Standard PCR with Taq polymerase, hot start Taq polymerase and proofreading polymerases
• qPCR with Taq polymerase and with hot start Taq polymerase
• qRT-PCR with Taq polymerase and with hot start Taq polymerase.

• an evolving intellectual property landscape
• new pricing strategies from established suppliers
• application to emerging research areas such as gene silencing, SNP genotyping and rapid, whole genome sequencing

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