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Nucleic Acid Separation Life Science Dashboard™ Series 1

Published by: Percepta Associates, Inc.

Published: Nov. 1, 2008 - 183 Pages


Table of Contents




Executive Summary

Key Findings and Implications

Nucleic Acid Separation Dashboard

Survey Methodology

Survey Invitation Text

Respondent Demographics

Frequency of Performance of Life Science Techniques

Frequency of Performance of Various Nucleic Acid Separation Techniques

Reaction Throughput and Market Segment Growth Rates

Respondent’s Stated Price Per Reaction

Total Market Size, Market Segment Sizes and Total Market Growth Rate

Matrix/Method Used to Separate Various Types of Nucleic Acids

Market Shares by Segment (Share of Mention)

Required Resolution for Nucleic Acid Separation Experiments

Required Throughput for Nucleic Acid Separation Experiments

Time Required for Nucleic Acid Separation Experiments

Customer Satisfaction and Interest in Switching Suppliers

Product Features that Influence Purchasing Decisions

Desired Changes to Nucleic Acid Separation Products

Survey Questionnaire

Figures and Tables

Figure 1: Respondent’s Place of Employment

Figure 2: Respondent’s Country/Region

Figure 3: Respondent’s Position/Job Title

Figure 4A: Respondent’s Areas of Expertise/Specialization

Figure 4B: Respondent’s Areas of Expertise/Specialization (Molecular Biology Excluded)

Figure 5: Number of Employees in Respondent’s Laboratories

Figure 6: Percentage of Respondents Performing Various Life Science Techniques at Least a Few Times per Year

Figure 7: Percentage of Respondents Performing Nucleic Acid Separation

Figure 8: Percentage of Respondents Performing Various Nucleic Acid Separation Methods at Least a Few Times per Year

Figure 9: Percentage of Respondents that Analyze Amplified DNA (Includes AFLP)

Figure 10: Percentage of Respondents that Analyze Restriction Digested DNA (Includes RFLP)

Figure 11: Percentage of Respondents that Analyze cRNA or Total RNA Quality

Figure 12: Percentage of Respondents that Analyze Small RNA Molecules

Figure 13: Percentage of Respondents that Analyze Synthetic DNA or RNA

Figure 14: Percentage of Respondents that Perform STR Analysis

Figure 15: Percentage of Respondents that Perform HLA Typing Analysis

Figure 16: Percentage of Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)

Figure 17: Percentage of Respondents that Perform Mutation Detection (Includes SSCP, DGGE)

Figure 18: Method/Matrix Used by Respondents that Analyze Amplified DNA (Includes AFLP)

Figure 19: Method/Matrix Used by Respondents that Analyze Restriction Digested DNA (Includes RFLP)

Figure 20: Method/Matrix Used by Respondents that Analyze cRNA or Total RNA Quality

Figure 21: Method/Matrix Used by Respondents that Analyze Small RNA MoleculesFigures

Figure 22: Method/Matrix Used by Respondents that Analyze Synthetic DNA or RNA

Figure 23: Method/Matrix Used by Respondents that Perform STR Analysis

Figure 24: Method/Matrix Used by Respondents that Perform HLA Typing Analysis

Figure 25: Method/Matrix Used by Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)

Figure 26: Method/Matrix Used by Respondents that Perform Mutation Detection (Includes SSCP, DGGE)

Figure 27: Respondent's Primary Supplier of Consumables for Pour-Your-Own Agarose Gels

Figure 28: Respondent's Primary Supplier of Instruments Used to Run Pour-Your-Own Agarose Gels

Figure 29: Respondent's Primary Supplier of Precast Agarose Gels

Figure 30: Respondent's Primary Supplier of Instruments Used to Run Precast Agarose Gels

Figure 31: Respondent's Primary Supplier of Consumables for Separation of Nucleic Acids by Capillary Electrophoresis

Figure 32:Respondent's Primary Supplier of Capillary Electrophoresis Instrumentation for Separation of Nucleic Acids

Figure 33: Respondent's Primary Supplier of Consumables for Microfluidics-Based Separation of Nucleic Acids

Figure 34: Respondent's Primary Supplier of Instrumentation for Microfluidics-Based Separation of Nucleic Acids

Figure 35: Respondent's Primary Supplier of Consumables Used to Run Polyacrylamide Gels

Figure 36: Respondent's Primary Supplier of Instruments Used to Run Polyacrylamide Gels

Figure 37: Resolution Required by Respondents that Analyze Amplified DNA (Includes AFLP)

Figure 38: Resolution Required by Respondents that Analyze Restriction Digested DNA (Includes RFLP)

Figure 39: Resolution Required by Respondents that Analyze cRNA or Total RNA Quality

Figure 40: Resolution Required by Respondents that Analyze Small RNA Molecules

Figure 41: Resolution Required by Respondents that Analyze Synthetic DNA or RNA

Figure 42: Resolution Required by Respondents that Perform STR Analysis

Figure 43: Resolution Required by Respondents that Perform HLA Typing Analysis

Figure 44: Resolution Required by Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)

Figure 45: Resolution Required by Respondents that Perform Mutation Detection (Includes SSCP, DGGE)

Figure 46: Separation Throughput Required by Respondents that Analyze Amplified DNA (Includes AFLP)

Figure 47: Separation Throughput Required by Respondents that Analyze Restriction Digested DNA (Includes RFLP)

Figure 48: Separation Throughput Required by Respondents that Analyze cRNA or Total RNA Quality

Figure 49: Separation Throughput Required by Respondents that Analyze Small RNA Molecules

Figure 50: Separation Throughput Required by Respondents that Analyze Synthetic DNA or RNA

Figure 51: Separation Throughput Required by Respondents that Perform STR Analysis

Figure 52: Separation Throughput Required by Respondents that Perform HLA Typing Analysis

Figure 53: Separation Throughput Required by Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)

Figure 54: Separation Throughput Required by Respondents that Perform Mutation Detection (Includes SSCP, DGGE)

Figure 55: Completion Time Required by Respondents that Analyze Amplified DNA (Includes AFLP)

Figure 56: Completion Time Required by Respondents that Analyze Restriction Digested DNA (Includes RFLP)

Figure 57: Completion Time Required by Respondents that Analyze cRNA or Total RNA Quality

Figure 58: Completion Time Required by Respondents that Analyze Small RNA Molecules

Figure 59: Completion Time Required by Respondents that Analyze Synthetic DNA or RNA

Figure 60: Completion Time Required by Respondents that Perform STR Analysis

Figure 61: Completion Time Required by Respondents that Perform HLA Typing Analysis

Figure 62: Completion Time Required by Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)

Figure 63: Completion Time Required by Respondents that Perform Mutation Detection (Includes SSCP, DGGE)

Figure 64: Percentage of Respondents That Have Switched Suppliers in the Last Six Months

Figure 65: Most Important Features of Products for Nucleic Acid Separation Experiments




Table 1: Respondent's Areas of Expertise/Specialization -Values for Figures 4A and 4B

Table 2: Frequency of Performance of Various Life Science Techniques

Table 3: Frequency of Co-Performance of Various Life Science Techniques

Table 4: Frequency of Performance of Nucleic Acid Separation Methods

Table 5: Frequency of Co-Performance of Life Science Techniques with Nucleic Acid Separation Methods

Table 6: Frequency of Co-Performance of Nucleic Acid Separation Methods with Life Science Techniques

Table 7: Median and Average Monthly Throughput for Nucleic Acid Separation Techniques

Table 8: Percentage of Respondents Separating Various Numbers of Nucleic Acid Samples (Lanes) Per Month

Table 9: Projected Growth in the Performance of Various Nucleic Acid Separation Techniques

Table 10: Median and Average Price Per Sample for Nucleic Acid Separation Products

Table 11: Estimated 2008 Global Market Size for Nucleic Acid Separation Product Categories

Table 12: Estimated 2009 Global Market Size for Nucleic Acid Separation Product Categories

Table 13: Matrix/Method Used to Separate Various Types of Nucleic Acids

Table 14: Market Share Leaders for Consumables for Pour-Your-Own Agarose Gels by Market Segment

Table 15: Market Share Leaders for Instruments for Pour-Your-Own Agarose Gels by Market Segment

Table 16: Market Share Leaders for Consumables for Precast Agarose Gels by Market Segment

Table 17: Market Share Leaders for Instruments for Precast Agarose Gels by Market Segment

Table 18: Market Share Leaders for Consumables for Capillary Electrophoresis by Market Segment

Table 19: Market Share Leaders for Instruments for Capillary Electrophoresis by Market Segment

Table 20: Market Share Leaders for Consumables for Microfluidics-Based Separation of Nucleic Acids by Market Segment

Table 21: Market Share Leaders for Instruments for Microfluidics-Based Separation of Nucleic Acids by Market Segment

Table 22: Market Share Leaders for Consumables for Polyacrylamide Gels by Market SegmentFigures

Table 23: Market Share Leaders for Instruments for Polyacrylamide Gels by Market Segment

Table 24: Market Share Leaders for Nucleic Acid Separation Products

Table 25: Resolution Required for Separation of Various Nucleic Acid Separation Methods

Table 26: Throughput Required for Various Nucleic Acid Separation Methods

Table 27: Completion Time Required for Various Nucleic Acid Separation Methods

Table 28: Percentage of Respondents Satisfied with Various Nucleic Acid Separation Products and Reasons for Dissatisfaction

Table 29: Most Important Features of Products for Nucleic Acid Separation Experiments

Abstract

The separation of nucleic acids is a necessary precursor to a wide range of life science research and diagnostic techniques. Familiar methods such as cloning/subcloning and Southern and Northern blotting frequently rely on the electrophoretic separation of nucleic acid fragments generated by amplification or restriction digestion.

Recently emerging methods such as gene silencing and miRNA analyses often hinge on the separation and sizing of short polynucleotides isolated from cell or tissue samples or synthesized in vitro. For some techniques, such as mutation detection or STR analysis, resolution in the range of a few bases may be required. For other methods, like standard cloning or Southern blotting, a resolution in the tens to hundreds of bases may suffice.

Percepta’s 2008 Nucleic Acid Separation Dashboard™ dives deeply into the characteristics of the market for nucleic acid separation products. This Dashboard reveals key market indicators for the nucleic acids separations market as a whole as well as for the following sub-segments:
  • Analysis of amplified DNA (includes AFLP)
  • Analysis of restriction digested DNA (includes RFLP)
  • Analysis of cRNA or total RNA quality
  • Analysis of small RNA molecules
  • Analysis of synthetic DNA or RNA
  • STR analysis
  • HLA typing analysis
  • Pulsed field gel electrophoresis (includes mapping)
  • Mutation detection (includes SSCP, DGGE)


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