Protein Purification Systems: Market Opportunities for Research Product Suppliers

Published by: BioInformatics, LLC

Published: Oct. 1, 2003 - 163 Pages

Table of Contents

Section 1. Executive Overview

1-1 Overview

Introduction

1-3 Objectives

1-4 Experimental Design

1-5 Product Mix

Technical

1-7 Throughput and Growth

1-9 Automation

1-11 Tagged and Untagged Proteins

1-13 Protein Purity

Products

1-15 Cell Lysis Buffers

1-16 Vectors

1-18 Purification Columns and Resins

1-20 Tag-Specific Antibodies

1-21 Cleavage of Protein Tags

Conclusion

1-23 Similarities Among Academia and Industry

1-24 Customer Preferences Along the Value Chain

1-29 Creating Customer Loyalty Along the Value Chain

Section 2. Study Methodology and Demographics

2-1 Objectives

2-1 Comments

2-5 Assumptions

2-5 Definition of Terms

2-5 Market Segment

2-6 Job Position

2-6 Geographic Region

2-6 Areas of Research

2-7 Questionnaire

Section 3. Presentation of Survey Data

Purification of Recombinant Protein

3-1 Systems used to express recombinant protein

3-4 Use of protein purification

3-5 Types of proteins most often purified

3-7 Primary reasons for purifying a certain type of

protein

3-8 Categories of proteins that are purified

3-9 Origin of proteins that are purified

3-10 Applications for which purified proteins are used

3-11 Typical number of purified protein preps performed per month

3-12 Predicted future change in purified protein preps

3-15 Average protein yield per prep

3-16 Final purity of protein preps on average

3-17 Problems and limitations encountered in protein purification

Techniques

3-18 Commercial cell lysis products used

3-20 Availability of flexible scaling in current system

3-21 Column chromatography methods used for purifying non-tagged proteins

Tagged Protein Purification

3-22 Tagged protein/purification methods used

3-23 Primary tagged protein/purification method used

3-24 Primary supplier of vectors

3-26 Satisfaction with specific features of vectors used

3-28 Overall satisfaction with vectors used

3-29 Primary supplier of purification resin/columns

3-31 Satisfaction with specific features of the purification resin/columns used

3-33 Overall satisfaction with the purification resin/columns used

3-34 Preferred supplier of primary antibodies against the tag

3-36 Percentage of time the tag is cleaved off from the protein

3-37 Proteases used when cleaving protein tags

3-38 Techniques used to monitor success/completeness of cleavage

Automation

3-39 Interest in automation of protein purification

3-43 Ideal number of preps per run in an automated protein purification system

3-44 Minimum yield of protein required per prep

Section 4. Tagged Protein Purification Systems Profile

4-1 Polyhistidine/metal affinity system (Respondents=247)

4-5 Glutathione S-transferase/glutathione affinity system (Respondents=106)

4-9 FLAG/antibody affinity system (Respondents=54)

4-13 HA Tag affinity system (Respondents=36)

Each profile includes:

• Primary supplier of vectors

• Satisfaction with specific features of vectors used

• Overall satisfaction with vectors used

• Primary supplier of purification resin/columns

• Satisfaction with specific features of the purification resin/columns used

• Overall satisfaction with the purification resin/columns used

Section 5. Appendices

5-1 Insights & Perspectives

5-18 Cross-Tabulations of Survey Data

5-35 Other Recent Publications

5-44 About BioInformatics

5-45 Our Valued Clients

Abstract

This report will help suppliers exploit opportunities to differentiate themselves from their competitors and develop more advanced protein purification tools

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