Immunohistochemistry Market Benchmarking Survey

Published by: BioInformatics, LLC

Published: Oct. 1, 2003 - 90 Pages

Table of Contents

Section 1. Executive Overview

1-1 Immunohistochemistry: An Indispensable Enabling Technique

1-1 Spotlight on IHC’s Role in Supporting Emerging Technologies

1-4 Market Opportunities

1-5 Future Outlook

Section 2. Study Methodology and Demographics

2-1 Objectives

2-1 Comments

2-3 Assumptions

2-3 Definition of Terms

2-4 Market Segment

2-4 Job Position

2-4 Geographic Region

2-4 Areas of Research

2-5 Questionnaire

Section 3. Presentation of Survey Data

Tissue/Sample Preparation and Handling

3-1 Preparation of tissue blocks/slides

3-3 Species from which tissues are analyzed by IHC

3-5 Types of cells or tissues on which IHC is performed

3-9 Sample preparation for IHC

3-13 Average number of hours spent fixing samples

3-14 Types of glass slide used for IHC analysis

3-15 Performance of an antigen retrieval step prior to performing an IHC assay

3-17 Performance of a protein digestion step prior to an IHC assay

3-19 Number of sections typically on each slide for IHC assays

3-20 Average number of individual IHC assays performed per week

Antigen Detection

3-21 Antigens most frequently detected by IHC analysis

3-23 Detection methods used to visualize IHC assay results

3-26 Types of primary antibody used

3-27 Types of secondary antibody used

3-29 Counterstaining of sample sections when performing IHC assays

3-31 Primary supplier of primary antibodies, secondary antibodies and detection reagents

Instrumentation and Hardware

3-35 Types and manufacturers of microscopes used

3-38 Types of cameras used to capture IHC assay results

3-39 Use of automatic slide stainers

Assay Requirements

3-41 Most important features of IHC detection systems

3-43 Usefulness of quantitative IHC

3-44 Performance/operational problems experienced in IHC experiments

3-45 Percentage of IHC experiments performed on specific types of antigens

3-46 Use of laser microdissection on tissue sections that have been analyzed by IHC

3-48 Area of research

Section 4. Appendices

4-1 Insights & Perspectives

4-2 Other Recent Publications

4-11 About BioInformatics

4-12 Our Valued Clients

Abstract

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