Protein Chromatography: Tools for Protein Expression and Purification : Market Research Report

Protein Chromatography: Tools for Protein Expression and Purification

Published by: Biocompare

Published: Jul. 10, 2006 - 62 Pages

I. Report Introduction
II. Market Overview
III. Industry Insights: a. Qiagen; b. Applied Biosystems
IV. Academic Assessment
V. Survey Introduction and Methodology
VI. Discussion of Survey Findings
VII. Conclusion/Recommendations
VIII. Appendix I: Questionnaire
IX. Appendix II: Presentation of Survey Data: a. Demographic Survey Data; b. Protein Chromatography Survey Data
Demographic Questions: a. Country; b. Institution Type; c. Title; d. Key Areas of Research or Work; e. Purchasing Authority
Protein Chromatography Questions: f. How would you characterize your use of chromatography techniques to purify proteins in your research or work?; g. Which of the following chromatography techniques do you use or plan to use?; h. What type of protein purification are you performing?; i. What are the primary applications of your purified protein? (check all that apply); j. Which of the following chromatography products do you use or plan to use?; k. What type of chromatography media do you use? (check all that apply); l. What company would you identify as your primary supplier of the chromatography media you use?; m. What affinity tags do you use? (check all that apply); n. What type(s) of recombinant protein expression system do you use? (check all that apply); o. What key challenges do you face with purifying your recombinant protein? (check all that apply); p. For the protein your work with most often, what is your typical yield for each prep?; q. Please estimate how many protein preps you perform in a month.; r. Which of the following chromatography systems do you use?; s. What brand of automated chromatography system do you primarily use?; t. Do you out-source protein custom services?; u. If yes, what are your primary needs?; v. Which of the following do you plan to purchase and what is your purchasing timeframe?; w. What are the three main criteria you would choose to make a purchasing decision of a chromatography system?; x. Are there any features not present on your current purification system that you would like to see available on future models? (open-ended)

Abstract

The 2006 Protein Chromatography Report is composed of a market overview and an introduction to the 2006 Protein Chromatography Survey, which contains questions about chromatography systems and related applications that researchers are using. The report also includes a discussion of the survey results and conclusions and recommendations drawn from both a market analysis of protein chromatography and the survey data. A new feature, academic assessment, presents an interview with an expert in protein chromatography and protein expression systems that rounds out this comprehensive report.

Biocompare spoke with Irina Neverova, Ph.D., adjunct professor of physiology at Queen’s University in Ontario, Canada, to discuss her perspective on proteomics and the advances in protein separation techniques. She describes the proteomics movement as “in a big transition period,” with the primary challenge being “pulling apart complex proteomes” in terms of the “different intrinsic properties” of the proteins and the span of their known concentrations reaching 1010. She predicts that multidimensional fractionation will aid researchers in the separation of proteins, provided that the columns and samples are optimized and that conditions are controlled. When queried about automation, Dr. Neverova commented that the pharmaceutical industry has made significant progress in this area, while academia is less focused on automation in general. She remarks that high resolution and sensitivities are important benchmarks that have not yet been achieved, but are essential for molecular diagnostics, such as the detection of biomarkers in a complex protein mixture such as plasma. Dr. Neverova predicts that high-performance liquid chromatography (HPLC), in addition to reversed-phase, affinity, and ion-exchange chromatography methods, will continue to evolve in terms of selectivity and miniaturization advances as well.

The market overview supports the opinions of Dr. Neverova as the protein chromatography market has continued to grow, notably in clinical and biomarker applications. The diverse selection of chromatographic techniques, such as capillary electrophoresis, HPLC, and ion-exchange, affinity, and two-dimensional chromatography, allows for significant advances in the improvements and innovations that lead to higher sensitivities, specificities, and reproducibilities in protein separation. At present, protein chromatography products bring in revenues surpassing $150 million and are expected to top $237 million by 20103. The entire liquid chromatography market is predicted to exceed $3 billion over the next 4 years.

Certain analytical systems are expected to outperform others, as the need for increased throughput and miniaturization increase, particularly in the pharmaceutical arena. HPLC and electrophoresis are expected to grow faster than other separation technologies. Niche separation products, such as chip-based microarrays and liquid chromatography/mass spectrometry (LC/MS) hybrid systems, are growing at over 15% annually. Such products are the result of technological innovations that drive improvements in resolution, speed, and sensitivity, of utmost importance in the growing clinical proteomics field.

The marketplace is crowded, however, and companies have had to strategize ways to stay one step ahead of their competition, including designing custom systems, offering value-based service and adequate training for sales personnel, increasing price-to-performance ratios, and continuing to innovate and differentiate their particular products and services. With the decreasing costs of instrumentation, profit margins are slim and the promise of huge profit margins in this product area will be difficult for vendors to realize.

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Protein Chromatography: Tools for Protein Expression and Purification

Table of Contents

Abstract