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Protein Microarrays: Technology Adoption & UtilizationPublished by: BioInformatics, LLC Published: Sep. 1, 2005 - 143 Pages Table of ContentsSection 1. Analysis and Interpretation of Survey Results Strategic Overview 1-1 Executive summary 1-2 Key findings Competing Technologies 1-4 Protein microarray adoption rates 1-5 Nonuser technology profile 1-6 Market segment technology preferences 1-7 Value of technology performance Experimental Parameters 1-9 Protein microarray content 1-10 Protein classes 1-11 Protein microarray density 1-12 Whole proteome arrays 1-13 Protein microarray format 1-14 Protein microarray throughput Market Dynamics: Protein Microarrays 1-16 Preferred suppliers of self-print protein microarray reagents and/or instrumentation 1-17 Protein microarray reagents and/or instrumentation performance attributes 1-18 Preferred suppliers of commercial protein microarrays 1-19 Commercial protein microarray performance attributes Market Dynamics: Protein Microarray Readers 1-21 Preferred suppliers of readers 1-22 Performance attributes Obstacles and Opportunities 1-24 Technology improvements Conclusions Section 2. Study Methodology and Demographics 2-1 Methodology 2-5 Definition of terms 2-6 Demographics 2-7 Questionnaire Section 3. Presentation of Survey Data Research Focus 3-1 Primary focus of research 3-3 Primary source of proteins 3-4 Proteomics-based technologies regularly employed 3-5 Current protein array use Future Plans 3-9 Cost per protein for alternate proteomics-based technologies 3-11 Willingness to pay more for wider dynamic range 3-12 Willingness to pay more for increased reproducibility 3-13 Willingness to pay more for increased sensitivity 3-14 Factors that would influence non-users of protein arrays to begin use or begin use sooner Experimental Parameters 3-16 Types of protein interactions studied 3-18 Types of applications for protein microarrays 3-20 Specific protein classes studied 3-22 Primary organism of interest for a whole proteome array 3-24 Proteins printed per array 3-26 How many replicates printed on arrays 3-28 Samples analyzed per array 3-32 Samples analyzed per month 3-33 Expected change in throughput per month 3-35 Microarray cost per protein 3-36 Willingness to pay more for arrays with a two-fold increase in proteins printed per array 3-37 Willingness to pay more for arrays with a ten-fold increase in proteins printed per array 3-38 Willingness to pay more for a whole proteome array 3-39 Most problematic step in protein microarray experiments Protein Microarray Printing 3-40 Use of commercial protein microarrays 3-41 Primary reason for using preferred type of microarrays 3-42 Suppliers of treated surfaces for protein microarrays 3-44 Primary supplier of reagents and instrumentation 3-46 Satisfaction with features of reagents and instrumentation from primary supplier 3-48 Overall satisfaction with reagents and instrumentation from primary supplier 3-49 Primary supplier of protein microarrays 3-51 Satisfaction with features of microarrays from primary supplier 3-53 Overall satisfaction with microarrays from primary supplier 3-54 Supplier factors in protein microarray purchasing decision Protein Microarray Detection 3-55 Primary brand of microarray reader used 3-57 Satisfaction with features of this microarray reader 3-59 Overall satisfaction with this microarray reader 3-60 Supplier factors in microarray reader purchasing decision Future Directions 3-61 Areas of improvement in protein microarray technologyDemographics Section 4. Appendices 4-1 Insights and Perspectives 4-10 Cross-Tabulations of Survey Data 4-21 Other Recent Publications 4-23 About BioInformatics 4-24 Our Valued Clients AbstractAs protein arrays have become more consistent and reliable, scientists have begun to incorporate them into their proteomics experiments. The technology's ability to detect protein expression more quickly and easily than traditional approaches has begun to transform the dream of characterizing an organism's entire collection of proteins (i.e., proteome) into reality through high throughput analysis. While there is tremendous excitement about the potential of protein arrays to further our understanding about protein function, there are also concerns over frustrating technical limitations.Based on a 39-question survey of scientists in the field of proteomics, Protein Microarrays: Technology Adoption & Utilization provides suppliers with insights into the types of products required to support this research. In order to benchmark the current state of the market for protein arrays, this report details researchers’ experimental parameters, current and future levels of sample throughput and choice of supplier. It reveals respondents’ satisfaction with their protein arrays, reagents and instrumentation, and determines the product attributes that are of the greatest importance, as well as areas where they perform above or below expectations. Additionally, the report explores why some proteomics researchers opt not to use protein arrays and what factors could influence scientists to begin using them.
By using this report to understand the actual experiences, needs and preferences of researchers, suppliers of protein arrays, arraying supplies and related equipment will be better able to make refinements to existing products to appeal to the diverse needs of their target market and extend the use of their protein array products into additional laboratories.
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