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The RNA Purification Market: Evaluating Competitive Dynamics & Establishing Benchmarks

Published by: BioInformatics, LLC

Published: Jun. 1, 2005 - 186 Pages


Table of Contents



Section 1. Analysis and Interpretation of Survey Results

Executive Summary

1-1 Introduction and Key Findings

1-2 Study Objectives

Market Profile: Uses and Applications of Purified RNA

1-4 Types of RNA

1-5 Applications

Experimental Design Parameters

1-7 Sample Source

1-8 Sample Size and Yield

1-9 Throughput

1-11 Research Focus

Market Dynamics: Users of Commercial Kits

1-13 RNA Purification Approaches

1-15 Preferred Suppliers

1-16 Product Performance Attributes

1-18 Understanding Customer Loyalty

1-19 Measuring Satisfaction and Loyalty

Market Dynamics: Users of Commercial Reagents

1-21 Preferred Suppliers

1-22 Product Performance Attributes

1-24 Understanding Customer Loyalty

1-25 Measuring Satisfaction and Loyalty

Market Dynamics: Users of Homebrew

1-27 Product Performance Attributes

1-28 Factors Driving Homebrew Use

Future Directions in the RNA Purification Market

1-30 High Throughput Robotics

1-31 Outsourcing Sample Purification

Conclusion




Section 2. Study Methodology and Demographics

2-1 Methodology

2-5 Definition of Terms

2-7 Demographics

2-8 Questionnaire




Section 3. Presentation of Survey Data

Usage and Applications

3-1 Use of RNA purification

3-3 Types of RNA purified/isolated for analysis

3-4 Applications for which purified RNA is used

3-5 Sources from which RNA is purified

3-6 Age of typical RNA sample purified from formalin-fixed, paraffin embedded tissue

3-7 Percentage of RNA samples purified from specific sources

3-8 Methods used to purify RNA

3-9 Methods used for cell/tissue disruption in RNA purification

3-12 Formats used for purifying RNA

3-13 Methods used to assess the quality of RNA

3-14 Minimum acceptable quality of RNA for use in downstream applications

3-17 Methods used to store purified RNA

3-18 Average number of different downstream applications for which each unique sample of RNA is utilized




RNA Purification Samples

3-19 Number of biological samples from which RNA is purified at one time

3-20 Number of biological samples from which RNA is purified per month

3-21 Expectation for the number of samples purified per month to change over the next twelve months

3-23 Amount of starting sample

3-26 Typical yield of total RNA

3-29 Percentage of samples purified using specific methods

3-30 Primary suppliers for both RNA purification kits and reagents




RNA Purification Kits and Reagents

3-34 Satisfaction with specific features of RNA purification kits/reagents from primary suppliers

3-36 Overall satisfaction with RNA purification kits/reagents from primary suppliers

3-37 Price paid per sample when purifying RNA using kits/reagents from primary suppliers




Supplier Performance

3-38 Reasons for using homebrew reagents for RNA purification

3-39 Length of time purchasing RNA purification kits/reagents from primary supplier

3-40 Opinion of primary supplier

3-41 Change in opinion of primary supplier during period of using their products

3-42 Likelihood to repurchase RNA purification kits/reagents from primary supplier

3-43 Likelihood to purchase other products from primary supplier




Future Directions

3-44 Use of robotic RNA purification system

3-46 Outsourcing samples for RNA purification

3-47 Supplier used for outsourced RNA purification samples




Section 4. Appendices

4-1 Insights and Perspectives

4-17 Cross-Tabulations of Survey Data

4-69 Other Recent Publications

4-71 About BioInformatics

4-72 Our Valued Clients

Abstract

RNA isolation/purification is a critical step in the preparation of biological samples for use in a multitude of downstream applications including real-time quantitative PCR, reverse transcription PCR, microarrays, Northern blot analysis, nuclease protection assays, RNA mapping, in vitro translation and cDNA library construction. The need for purified RNA in so many experiments across several scientific disciplines has quickly made RNA purification an indispensable tool for both academic and industrial scientists, and as such, a large market of RNA purification products - including both commercial kits and reagents - has developed.

One significant challenge facing the field of RNA purification is the difficulty of extracting RNA from different types of cells and tissues; a related challenge is sample scarcity, especially in a clinical setting. The heterogeneity of RNA attributes across different organisms and cell types adds additional complexity for suppliers - forcing them to design a range of sample-dependent offerings. In such a product landscape of kits and reagents applicable only to specific organisms, cell and/or tissue types, suppliers find themselves competing for share across a very fractured user base. As a result of these and other challenges, product offerings for RNA purification face performance mandates around yield and sample stability.

To gain a better understanding of the RNA purification techniques currently used, The RNA Purification Market: Evaluating Competitive Dynamics & Establishing Benchmarks provides suppliers with insights into the types of products required to support this work. Based on a 34-question survey of 813 scientists who currently purify RNA, this report benchmarks the current market by comprehensively reflecting researchers' experimental parameters and uses for purified RNA, current and future levels of sample throughput and the particular purification methods employed. In addition, it provides a closer look at two directions in which the RNA purification market could be moving-outsourcing samples for purification and the use of robotics for high throughput purification.

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