Advances in biomedical instrumentation are enabling a new perspective that could not have been achieved even a decade ago. Mass spectrometry is the most significant and far reaching of these developments, allowing the generation of data in the medical sciences that brings new insights into critical questions. This report analyzes:
- Physical principles and analytical applications of mass spectrometry
- The role of mass spectrometry in genomics, proteomics, metabolomics, and lipidomics
- Advances in mass spectrometry instrumentation and methods
- Current and emerging applications of mass spectrometry
- Future prospects for the mass spectrometry industry
Also included are interviews with mass spectrometry experts in the academic and private sectors
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- Executive Summary
- Chapter 1
INTRODUCTION
- 1.1. Scientific Background
- History of MS
- Physical Principles
- Analytical Applications
- 1.2. The Role of Mass Spectrometry in Genomics/ Proteomics/ Metabolomics/ Lipidomics
- 1.3. Conclusions: A Versatile Analytical Tool
- Chapter 2
MASS SPECTROMETRY INSTRUMENTS
- 2.1. Electrospray Ionization (ESI)
- 2.2. Matrix-Assisted Laser Desorption/Ionization (MALDI)
- 2.3. SELDI-TOF
- 2.4. Mass Analyzers
- Quadrupole Mass Filter
- Quadrupole Ion Trap
- Gas Chromatograph-Quadrupole Mass Spectrometer
- Time-of-Flight (TOF) Mass Analyzer
- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Tandem Mass Spectrometer (MALDI-TOF/TOF)
- Fourier-Transform Ion Cyclotron Resonance Mass Analyzer
- Hybrid Instruments
- Liquid Chromatography (LC)
- Gas Chromatography (GC)
- LC/MS
- Liquid Chromatography Combined with Tandem Mass Spectrometry (LC/MS/MS)
- LTQ Orbitrap and Synapt G2 Systems
- Magnetic Sector MS
- 2.5. Fragmentation Methods
- Tandem Mass Spectrometry MS/MS/MS, MS3, MSn
- Ion Trap
- Isotope-Coded Affinity Tagging (ICAT)
- Isobaric Tab for Relative and Absolute Quantitation (iTRAQ)
- Stable Isotope Labeling of Amino Acids in Cell Culture (SILAC)
- Tandem Mass Spectrometry (MS/MS)
- Collision-Induced Dissociation (CID)
- Electron Capture Dissociation (ECD)
- Electron Transfer Dissociation (ETD)
- Chapter 3
INSTRUMENTS USED IN CONJUNCTION WITH MASS SPECTROMETRY
- 3.1. Two-Dimensional Gel Electrophoresis
- 3.2. Two-Hybrid Analysis
- 3.3. Protein Microarrays
- 3.4. Protein Database Search Engines
- Chapter 4
APPLICATIONS OF MASS SPECTROMETRY TECHNOLOGY
- 4.1. Proteomics
- Early Investigations Fail to Deliver
- A Re-evaluation of the Discovery Process
- Multiplex Assays for Proteins
- The Problem of Dynamic Range
- MudPIT: A Powerful New Discovery Tool
- 4.2. Genomics
- New Sequencing Technologies Drive Genomics Advances
- Findings Arise From Genome Studies
- Personalized Cancer Therapies
- 4.3. Metabolomics
- 4.4. Lipidomics
- 4.5. Environmental Monitoring
- 4.6. Forensics
- 4.7. Sampling
- 4.8. Sample Preparation
- 4.9. Analysis of Impurities Using Mass Spec
- Chapter 5
CONCLUSIONS, FUTURE PROSPECTS
- 5.1. Systems Biology and Mass Spectrometry
- 5.2. Roadblocks and Challenges in Pursuit of Systems Biology
- 5.3. A New Perspective on Mass Spectrometry Instrumentation
- 5.4. The Mass Spec Industry Today
- 5.5. Into the Blue, with Caveats
- Chapter 6
INTERVIEWS WITH ACADEMIC AND PRIVATE SECTOR EXPERTS
- 6.1. Dr. Ruedi Aebersold, Professor, Institute of Molecular Systems Biology, Eidgenössische Technische Hochschule Zürich
- 6.2. Dr. R. Graham Cooks, PhD, Professor, Purdue University, Lafayette, IN
- 6.3. Dr. Srinivas Iyer, Research Scientist, Los Alamos National Laboratory, Los Alamos, NM
- 6.4. Dr. Vathany Kulasingam, PhD, Clinical Biochemist and Assistant Professor, Department of Clinical Biochemistry, University of Toronto
- 6.5. Emanuel F. Petricoin, PhD, Professor and Co-Director, Center for Applied Proteomics and Molecular Medicine, George Mason University
- 6.6. Dr. Ralph Schiess, Institute of Molecular Systems Biology, ETH Zürich
- 6.7. Dr. Ravinder Singh, PhD, Director, Mass Spectrometry Facility, The Mayo Clinic, Rochester, MN
- 6.8. Dr. Klaus Weinberger, CSO, BIOCRATES Life Sciences
- References
- Company Index
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